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I have a USB driver that will not load on Vista. I believe it is due to the usbser.sys not being present in Vista. I am using the following inf file.

; Windows USB SID Setup File
; Copyright © 2000 Microsoft Corporation
; Copyright © 2005 Plescon Ltd

[Version]
Signature="$CHICAGO$"
Class=Ports
ClassGuid={4D36E978-E325-11CE-BFC1-08002BE10318}
Provider=%MCHP%
LayoutFile=layout.inf
DriverVer=08/17/2001,5.1.2600.0

[Manufacturer]
%MFGNAME%=DeviceList

[DestinationDirs]
DefaultDestDir=12

[DeviceList]
%DESCRIPTION%=DriverInstall, USB\VID_ffff&PID_32a3

;------------------------------------------------------------------------------
; Windows 2000/XP Sections
;------------------------------------------------------------------------------

[DriverInstall]
CopyFiles=DriverCopyFiles
AddReg=DriverInstall.AddReg

[DriverCopyFiles]
usbser.sys

[DriverInstall.AddReg]
HKR,,DevLoader,,*ntkern
HKR,,NTMPDriver,,"usbser.sys"
HKR,,EnumPropPages32,,"MsPorts.dll,SerialPortPropPageProvider"

[DriverInstall.HW]
AddReg = DriverInstall.AddRegHW

[DriverInstall.AddRegHW]
HKR,,PortName,0x00000020,"COM4"

[DriverInstall.Services]
AddService=psp_usbser, 0x00000002, DriverService

[DriverService]
DisplayName=%SERVICE%
ServiceType=1
StartType=3
ErrorControl=1
ServiceBinary=%12%\usbser.sys

;------------------------------------------------------------------------------
; String Definitions
;------------------------------------------------------------------------------

[Strings]
MCHP="Plescon Ltd"
MFGNAME="Plescon Ltd"
DESCRIPTION="USB SID"
SERVICE="USB Scanner Controller"

Can anyone advise on where to go from here?

that file is part of your motherboard chipset drivers.

if you have updated to vista please load the vista drivers for your motherboard.